human chondrocyte propagation and

maintenance................................................. 163

porcine trachea collection................................. 162

repopulation, decellularized tracheal ECM-based

porcine bio-scaffold..................................... 164

tracheal segments ....................................................164

Tracheal transplantation ...............................................167

Triton X-100 ......................................................... 73, 162,

171–173, 177–179, 249, 254

U

Umbilical cord mesenchymal stem cells (UCMSCs)

cell culture, medium, replenishment, and growth

monitoring.......................................... 119–121

cell culture reagents ....................................... 114–115

cell harvesting................................................. 122–123

cell quality assessment-related reagents .................116

3D FloTrix^® digest solution...................................116

3D FloTrix^® vivaSPIN bioreactor................. 116–118

3D TableTrix^® microcarrier tablets .............. 115–116

inoculation on ................................................ 118–119

three-dimension (3D) dynamic cell culture ..........114

tissue engineering and cell-based therapies ...........113

Uniaxial stretching...................................... 136, 137, 143

W

Wave-assisted agitation .................................................152

Working cell bank (WCB) .............................................. 89

WST-1 proliferation assay.................................... 213–215

BIOREACTORS IN STEM CELL BIOLOGY: METHODS AND PROTOCOLS

Index ²⁷³